Protocol for induction and study of DNA double-strand breaks in mammalian cells using PALM microdissection and expansion microscopy
Summary: We present a protocol to induce localized DNA double-strand breaks (DSBs) in mammalian monolayer cells, using the PALM MicroBeam microdissector, followed by high-resolution visualization through expansion microscopy (ExM). We describe steps for laser microirradiation, immunostaining, and ge...
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| Asıl Yazarlar: | , , , , , , , , |
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| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
Elsevier
2025-09-01
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| Seri Bilgileri: | STAR Protocols |
| Konular: | |
| Online Erişim: | http://www.sciencedirect.com/science/article/pii/S2666166725003442 |
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