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Protocol for induction and study of DNA double-strand breaks in mammalian cells using PALM microdissection and expansion microscopy

Summary: We present a protocol to induce localized DNA double-strand breaks (DSBs) in mammalian monolayer cells, using the PALM MicroBeam microdissector, followed by high-resolution visualization through expansion microscopy (ExM). We describe steps for laser microirradiation, immunostaining, and ge...

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Principais autores: Fernando Luna-Maldonado, Diego Oliva-Rico, Alejandro López-Saavedra, Aquetzalli Arenas-Hernández, Clementina Castro-Hernández, Juan Eduardo Rodriguez Gatica, Eloy-Andres Pérez-Yepez, José Díaz-Chávez, Luis A. Herrera
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2025-09-01
Serier:STAR Protocols
Fag:
Cell Biology
Cancer
Microscopy
Online adgang:http://www.sciencedirect.com/science/article/pii/S2666166725003442
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