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Long undecoded transcript isoform (LUTI) detection in meiotic budding yeast by direct RNA and transcript leader sequencing

Summary: LUTIs (Long Undecoded Transcript Isoforms) are 5′-extended and poorly translated mRNAs that can downregulate transcription from promoters more proximal to a gene’s coding sequence (CDS). In this protocol, polyA RNA is extracted from budding yeast cells undergoing highly synchronized meiosis...

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Autori principali: Amy Tresenrider, Minghao Chia, Folkert J. van Werven, Elçin Ünal
Natura: Artigo
Lingua:Inglês
Pubblicazione: Elsevier 2022-03-01
Serie:STAR Protocols
Soggetti:
Bioinformatics
Sequence analysis
Genetics
Genomics
Sequencing
Model Organisms
Accesso online:http://www.sciencedirect.com/science/article/pii/S2666166722000259
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