Long undecoded transcript isoform (LUTI) detection in meiotic budding yeast by direct RNA and transcript leader sequencing

Summary: LUTIs (Long Undecoded Transcript Isoforms) are 5′-extended and poorly translated mRNAs that can downregulate transcription from promoters more proximal to a gene’s coding sequence (CDS). In this protocol, polyA RNA is extracted from budding yeast cells undergoing highly synchronized meiosis...

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Auteurs principaux:	Amy Tresenrider, Minghao Chia, Folkert J. van Werven, Elçin Ünal
Format:	Artigo
Langue:	Inglês
Publié:	Elsevier 2022-03-01
Collection:	STAR Protocols
Sujets:	Bioinformatics Sequence analysis Genetics Genomics Sequencing Model Organisms
Accès en ligne:	http://www.sciencedirect.com/science/article/pii/S2666166722000259
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