Evaluation of guide-free Cas9-induced genomic damage and transcriptome changes in pig embryos

Cas9 protein without sgRNAs can induce genomic damage at the cellular level in vitro. However, whether the detrimental effects occur in embryos after Cas9 treatment remains unknown. Here, using pig embryos as subjects, we observed that Cas9 protein transcribed from injected Cas9 mRNA can persist unt...

Fuld beskrivelse

Bibliografiske detaljer

Principais autores:	Weikai Ge, Xiaozhu Zhao, Shixue Gou, Qin Jin, Fangbing Chen, Zhen Ouyang, Chengdan Lai, Tao Cui, Baoyi Mai, Sijia Lu, Kexin Zhong, Yanhui Liang, Tao Chen, Han Wu, Nan Li, Yinghua Ye, Liangxue Lai, Kepin Wang
Format:	Artigo
Sprog:	Inglês
Udgivet:	Elsevier 2023-12-01
Serier:	Molecular Therapy: Nucleic Acids
Fag:	MT: RNA/DNA Editing guide-free Cas9 germline genome editing genomic damage transcriptome changes pig embryo
Online adgang:	http://www.sciencedirect.com/science/article/pii/S2162253123002536
Tags:	Tilføj Tag Ingen Tags, Vær først til at tagge denne postø!