Optimized Protocol for High-Quality RNA Extraction from Grape Berry Skins Using Sorbitol Pre-Wash

Extracting high-quality RNA from grape (<i>Vitis Vinifera</i>) berry skins is challenging due to their high levels of polysaccharides, phenolic compounds, sugars, and organic acids, which can negatively impact RNA purity and yield. Indeed, polyphenols can bind to RNA, polysaccharides may co-precipit...

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Principais autores:	Annalisa Prencipe, Antonella Salerno, Margherita D’Amico, Antonio Domenico Marsico, Mario Ventura, Riccardo Velasco, Maria Francesca Cardone, Carlo Bergamini, Flavia Angela Maria Maggiolini
格式:	Artigo
語言:	Inglês
出版:	MDPI AG 2025-03-01
叢編:	Plants
主題:	<i>Vitis vinifera</i> RNA sorbitol extraction protocol
在線閱讀:	https://www.mdpi.com/2223-7747/14/7/988
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