A Simplified and Efficient Method for Himar-1 Transposon Sequencing in Bacteria, Demonstrated by Creation and Analysis of a Saturated Transposon-Mutant Library in <named-content content-type="genus-species">Mycobacterium abscessus</named-content>

ABSTRACT We present a technically simple, easy-to-perform method for generating the genomic libraries for Himar-1 transposon site sequencing (Tn-seq). In addition to being simpler than present methods in the technical aspect, it also allows more robust and straightforward identification of the inser...

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Autors principals:	Mark Foreman, Moran Gershoni, Daniel Barkan
Format:	Artigo
Idioma:	Inglês
Publicat:	American Society for Microbiology 2020-10-01
Col·lecció:	mSystems
Matèries:	transposon genomics bacterial genetics Mycobacterium abscessus bioinformatics mycobacteria
Accés en línia:	https://journals.asm.org/doi/10.1128/mSystems.00976-20
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