A Simplified and Efficient Method for Himar-1 Transposon Sequencing in Bacteria, Demonstrated by Creation and Analysis of a Saturated Transposon-Mutant Library in <named-content content-type="genus-species">Mycobacterium abscessus</named-content>
ABSTRACT We present a technically simple, easy-to-perform method for generating the genomic libraries for Himar-1 transposon site sequencing (Tn-seq). In addition to being simpler than present methods in the technical aspect, it also allows more robust and straightforward identification of the inser...
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| Автори: | , , |
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| Формат: | Artigo |
| Мова: | Inglês |
| Опубліковано: |
American Society for Microbiology
2020-10-01
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| Серія: | mSystems |
| Предмети: | |
| Онлайн доступ: | https://journals.asm.org/doi/10.1128/mSystems.00976-20 |
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