A Simplified and Efficient Method for Himar-1 Transposon Sequencing in Bacteria, Demonstrated by Creation and Analysis of a Saturated Transposon-Mutant Library in <named-content content-type="genus-species">Mycobacterium abscessus</named-content>
ABSTRACT We present a technically simple, easy-to-perform method for generating the genomic libraries for Himar-1 transposon site sequencing (Tn-seq). In addition to being simpler than present methods in the technical aspect, it also allows more robust and straightforward identification of the inser...
Enregistré dans:
| Auteurs principaux: | , , |
|---|---|
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
American Society for Microbiology
2020-10-01
|
| Collection: | mSystems |
| Sujets: | |
| Accès en ligne: | https://journals.asm.org/doi/10.1128/mSystems.00976-20 |
| Tags: |
Pas de tags, Soyez le premier à ajouter un tag!
|