Introduction of plasmid encoding for rare tRNAs reduces amplification bias in phage display biopanning

Amplification bias is a major hurdle in phage display protocols because it imparts additional, unintended selection pressure beyond binding to the desired target. One potential source of amplification bias is the inherent lack of codon optimization that occurs within phage display libraries. Here we...

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Bibliografiska uppgifter

Huvudupphov:	Benjamin J. Umlauf, Michael J. McGuire, Kathlynn C. Brown
Materialtyp:	Artigo
Språk:	Inglês
Utgiven:	Taylor & Francis Group 2015-02-01
Serie:	BioTechniques
Ämnen:	phage display amplification bias codon bias peptide combinatorial peptide libraries biopanning
Länkar:	https://www.future-science.com/doi/10.2144/000114256
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