Introduction of plasmid encoding for rare tRNAs reduces amplification bias in phage display biopanning

Amplification bias is a major hurdle in phage display protocols because it imparts additional, unintended selection pressure beyond binding to the desired target. One potential source of amplification bias is the inherent lack of codon optimization that occurs within phage display libraries. Here we...

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Prif Awduron:	Benjamin J. Umlauf, Michael J. McGuire, Kathlynn C. Brown
Fformat:	Artigo
Iaith:	Inglês
Cyhoeddwyd:	Taylor & Francis Group 2015-02-01
Cyfres:	BioTechniques
Pynciau:	phage display amplification bias codon bias peptide combinatorial peptide libraries biopanning
Mynediad Ar-lein:	https://www.future-science.com/doi/10.2144/000114256
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