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Capillary Zone Electrophoresis with Light-Emitting Diode-Induced Fluorescence Detection for the Analysis of Monoclonal Antibodies: Detector Optimization through Design of Experiments and Comparison to UV Detection

Capillary zone electrophoresis (CZE) is an important technique for the analysis of monoclonal antibodies (mAbs). A recently released light-emitting diode (LED)-induced fluorescence (LEDIF) detector equipped with a 275 nm LED for the detection of proteins through their native fluorescence was used in...

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Hlavní autoři: Holger Zagst, Sophie Hartung, Dina-Mareike Menges, Antonia Wittmann, Hermann Wätzig
Médium: Artigo
Jazyk:Inglês
Vydáno: MDPI AG 2023-05-01
Edice:Separations
Témata:
capillary zone electrophoresis
monoclonal antibody
LED-induced fluorescence
design of experiments
charge heterogeneity
On-line přístup:https://www.mdpi.com/2297-8739/10/5/320
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