Multi-Enzymatic Cascades in the Synthesis of Modified Nucleosides: Comparison of the Thermophilic and Mesophilic Pathways

A comparative study of the possibilities of using ribokinase → phosphopentomutase → nucleoside phosphorylase cascades in the synthesis of modified nucleosides was carried out. Recombinant phosphopentomutase from <i>Thermus thermophilus</i> HB27 was obtained for the first time: a strain producing a s...

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Main Authors:	Ilja V. Fateev, Maria A. Kostromina, Yuliya A. Abramchik, Barbara Z. Eletskaya, Olga O. Mikheeva, Dmitry D. Lukoshin, Evgeniy A. Zayats, Maria Ya. Berzina, Elena V. Dorofeeva, Alexander S. Paramonov, Alexey L. Kayushin, Irina D. Konstantinova, Roman S. Esipov
Format:	Artigo
Language:	Inglês
Published:	MDPI AG 2021-04-01
Series:	Biomolecules
Subjects:	purine nucleoside phosphorylase biocatalysis enzyme cascade expression of recombinant enzymes
Online Access:	https://www.mdpi.com/2218-273X/11/4/586
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