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Expression and Purification of the Thermus thermophilus Argonaute Protein

The Argonaute protein of Thermus thermophilus (TtAgo) has recently been studied in detail. For its in vitro characterization, TtAgo was purified after heterologous expression in Escherichia coli (E. coli). As TtAgo expression is toxic, a tightly controlled system was used for protein expression. The...

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書誌詳細
主要な著者: Daan Swarts, Matthijs Jore, John Oost
フォーマット: Artigo
言語:Inglês
出版事項: Bio-protocol LLC 2014-10-01
シリーズ:Bio-Protocol
オンライン・アクセス:https://bio-protocol.org/en/bpdetail?id=1253&type=0
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