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Expression and Purification of the Thermus thermophilus Argonaute Protein

The Argonaute protein of Thermus thermophilus (TtAgo) has recently been studied in detail. For its in vitro characterization, TtAgo was purified after heterologous expression in Escherichia coli (E. coli). As TtAgo expression is toxic, a tightly controlled system was used for protein expression. The...

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Bibliografiske detaljer
Principais autores: Daan Swarts, Matthijs Jore, John Oost
Format: Artigo
Sprog:Inglês
Udgivet: Bio-protocol LLC 2014-10-01
Serier:Bio-Protocol
Online adgang:https://bio-protocol.org/en/bpdetail?id=1253&type=0
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