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Effect of unlabeled helper probes on detection of an RNA target by bead-based sandwich hybridization

Unlabeled helper oligonucleotides assisting a bead-based sandwich hybridization assay were tested for the optimal placement of the capture and detection probes. The target used was a full-length in vitro synthesized mRNA molecule. Helper probes complementary to regions adjacent to the binding site o...

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Autors principals: Kim Bundvig Barken, Magdalena Gabig-Ciminska, Anders Holmgren, Søren Molin
Format: Artigo
Idioma:Inglês
Publicat: Taylor & Francis Group 2004-01-01
Col·lecció:BioTechniques
Accés en línia:https://www.future-science.com/doi/10.2144/04361RR03
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