Effect of unlabeled helper probes on detection of an RNA target by bead-based sandwich hybridization
Unlabeled helper oligonucleotides assisting a bead-based sandwich hybridization assay were tested for the optimal placement of the capture and detection probes. The target used was a full-length in vitro synthesized mRNA molecule. Helper probes complementary to regions adjacent to the binding site o...
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| Principais autores: | , , , |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Taylor & Francis Group
2004-01-01
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| 叢編: | BioTechniques |
| 在線閱讀: | https://www.future-science.com/doi/10.2144/04361RR03 |
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