Ethylenediaminetetraacetic Acid (EDTA)-Decalcified, Formalin-Fixed Paraffin-Embedded (FFPE) Tumor Tissue Shows Comparable Quality and Quantity of DNA to Non-Decalcified Tissue in Next-Generation Sequencing (NGS)

<b>Background:</b> Bone tissue decalcification is essential for histopathological evaluation, but conventional methods using inorganic acids degrade nucleic acids, limiting molecular testing. EDTA is known to better preserve DNA, but its suitability for next-generation sequencing (NGS) in clinical s...

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Bibliographic Details

Main Authors:	Francis Hong Xin Yap, Jen-Hwei Sng, Jeremy Wee Kiat Ng, Hanis Abdul Kadir, Pei Yi Chan, Timothy Kwang Yong Tay
Format:	Artigo
Language:	Inglês
Published:	MDPI AG 2025-09-01
Series:	Journal of Molecular Pathology
Subjects:	Ethylenediaminetetraacetic Acid (EDTA) next-generation sequencing (NGS) decalcification
Online Access:	https://www.mdpi.com/2673-5261/6/3/21
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