pTripleTREP – A vector for tightly controlled expression and purification of virulence factors in Staphylococcus aureus

Abstract Background Recombinant proteins facilitate and contribute to detailed studies of the virulence mechanisms and pathophysiology of the major human pathogen Staphylococcus aureus. Of particular interest are secreted virulence factors. However, due to their potential toxicity and specific post-...

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Bibliografske podrobnosti

Principais autores:	Hannes Wolfgramm, Christopher Saade, Marco Harms, Larissa M. Busch, Josephine Lange, Maximilian Schedlowski, Kristin Surmann, Manuela Gesell Salazar, Christian Hentschker, Leif Steil, Stephan Michalik, Uwe Völker, Alexander Reder
Format:	Artigo
Jezik:	Inglês
Izdano:	BMC 2025-05-01
Serija:	Microbial Cell Factories
Teme:	Homologous expression Protein purification Expression vector Staphylococcus aureus Twin-Strep-tag Tetracycline-inducible promoter
Online dostop:	https://doi.org/10.1186/s12934-025-02736-7
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