pTripleTREP – A vector for tightly controlled expression and purification of virulence factors in Staphylococcus aureus

Abstract Background Recombinant proteins facilitate and contribute to detailed studies of the virulence mechanisms and pathophysiology of the major human pathogen Staphylococcus aureus. Of particular interest are secreted virulence factors. However, due to their potential toxicity and specific post-...

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Principais autores:	Hannes Wolfgramm, Christopher Saade, Marco Harms, Larissa M. Busch, Josephine Lange, Maximilian Schedlowski, Kristin Surmann, Manuela Gesell Salazar, Christian Hentschker, Leif Steil, Stephan Michalik, Uwe Völker, Alexander Reder
Formato:	Artigo
Idioma:	Inglês
Publicado:	BMC 2025-05-01
Series:	Microbial Cell Factories
Assuntos:	Homologous expression Protein purification Expression vector Staphylococcus aureus Twin-Strep-tag Tetracycline-inducible promoter
Acceso en liña:	https://doi.org/10.1186/s12934-025-02736-7
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