Improved next-generation sequencing pre-capture library yields and sequencing parameters using on-bead PCR

Tumor DNA sequencing results can have important clinical implications. However, its use is often limited by low DNA input, owing to small tumor biopsy size. To help overcome this limitation we have developed a simple improvement to a commonly used next-generation sequencing (NGS) capture-based libra...

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Autors principals:	Christopher R McEvoy, Timothy Semple, Bhargavi Yellapu, David Y Choong, Huiling Xu, Gisela Mir Arnau, Andrew P Fellowes, Stephen B Fox
Format:	Artigo
Idioma:	Inglês
Publicat:	Taylor & Francis Group 2020-01-01
Col·lecció:	BioTechniques
Matèries:	DNA library next-generation sequencing solid-phase reversible immobilization beads
Accés en línia:	https://www.future-science.com/doi/10.2144/btn-2019-0059
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