Rates of homology directed repair of CRISPR-Cas9 induced double strand breaks are lower in naïve compared to primed human pluripotent stem cells

Gene editing in human pluripotent stem cells (hPSC) is a powerful tool for understanding biology, for drug discovery and gene therapy. Naïve hPSC have been suggested to be superior for gene editing compared to conventional ‘primed’ hPSC. Using droplet digital PCR, we uncover the kinetics of Cas9-ind...

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Principais autores:	Benjamin T. Dodsworth, Klas Hatje, Claas Aiko Meyer, Rowan Flynn, Sally A. Cowley
格式:	Artigo
语言:	Inglês
出版:	Elsevier 2020-07-01
丛编:	Stem Cell Research
主题:	Human Naïve Ground state Pluripotent Stem cell Pluripotency
在线阅读:	http://www.sciencedirect.com/science/article/pii/S1873506120301537
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