Rates of homology directed repair of CRISPR-Cas9 induced double strand breaks are lower in naïve compared to primed human pluripotent stem cells

Gene editing in human pluripotent stem cells (hPSC) is a powerful tool for understanding biology, for drug discovery and gene therapy. Naïve hPSC have been suggested to be superior for gene editing compared to conventional ‘primed’ hPSC. Using droplet digital PCR, we uncover the kinetics of Cas9-ind...

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Bibliografske podrobnosti

Principais autores:	Benjamin T. Dodsworth, Klas Hatje, Claas Aiko Meyer, Rowan Flynn, Sally A. Cowley
Format:	Artigo
Jezik:	Inglês
Izdano:	Elsevier 2020-07-01
Serija:	Stem Cell Research
Teme:	Human Naïve Ground state Pluripotent Stem cell Pluripotency
Online dostop:	http://www.sciencedirect.com/science/article/pii/S1873506120301537
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