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Estimation of pyrazinamidase activity using a cell-free In vitro synthesis of pnca and its association with pyrazinamide susceptibility in Mycobacterium tuberculosis

Background: The main mechanism of resistance to PZA in Mycobacterium tuberculosis relies on mutations on its pyrazinamidase/nicotinamidase. Recently, a rapid colorimetric test relying on the PCR-based in vitro-synthesized-PZase assay has been reported for PZase activity determination from clinical M...

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Principais autores: Daniel Rueda, Christine Bernard, Lucas Gandy, Estelle Capton, Rachid Boudjelloul, Florence Brossier, Nicolas Veziris, Mirko Zimic, Wladimir Sougakoff
Formato: Artigo
Idioma:Inglês
Publicado: Wolters Kluwer Medknow Publications 2018-01-01
Series:International Journal of Mycobacteriology
Assuntos:
Cell-free expression
Mycobacterium tuberculosis
PncA
pyrazinamidase
pyrazinamide
resistance
tuberculosis
Acceso en liña:http://www.ijmyco.org/article.asp?issn=2212-5531;year=2018;volume=7;issue=1;spage=16;epage=25;aulast=Rueda
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