Protocol for qualitative analysis of lysosome immunoprecipitation from patient-derived glioblastoma stem-like cells

Summary: Lysosomes are critical for the sustenance of glioblastoma stem-like cells (GSCs) properties. We present a protocol to enrich and purify lysosomes from patient-derived GSCs in culture. We describe the steps required to stably express a tagged lysosomal protein in GSCs, mechanically lyse cell...

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Bibliographic Details

Main Authors:	Clément Maghe, Julie Gavard
Format:	Artigo
Language:	Inglês
Published:	Elsevier 2024-06-01
Series:	STAR Protocols
Subjects:	Cell Biology Cell culture Cell separation/fractionation Cancer Molecular/Chemical Probes Protein Biochemistry
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166724002867
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