Scalable Production of Transfection-Grade Plasmid DNA by Liquid–Liquid Extraction Without Chromatography: Application to CAR-T Vector Packaging

The growing demand for high-quality plasmid DNA (pDNA) in cell and gene therapy, including chimeric antigen receptor T-cell therapy (CAR-T) manufacturing, is constrained by the limited availability of transfection-grade plasmids. This study describes a practical, scalable, and cost-effective acidic...

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Bibliografische Detailangaben

Hauptverfasser:	Gaziza Nigmatulla, Aitolkyn Kydyrbayeva, Tolganay Kulatay, Gulzat Zauatbayeva, Bakytkali Ingirbay, Lyazzat Sagyndykova, Viktoriya Keyer, Dinara Zharlyganova, Maral Zhumabekova, Alexandr V. Shustov
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	MDPI AG 2026-08-01
Schriftenreihe:	Bioengineering
Schlagworte:	plasmid DNA purification acidic phenol extraction lentiviral vector production CAR-T-cell therapy transfection-grade plasmid cost-effective manufacturing
Online-Zugang:	https://www.mdpi.com/2306-5354/13/8/939
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