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Protocol to measure and analyze protein interactions in mammalian cells using bioluminescence resonance energy transfer

Summary: Bioluminescence resonance energy transfer (BRET) allows to quantitate protein interactions in intact cells. Here, we present a protocol for measuring BRET due to transient interactions of oncogenic K-RasG12V in plasma membrane nanoclusters of HEK293-EBNA cells. We describe steps for seeding...

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Principais autores: Carla Jane Duval, Candy Laura Steffen, Karolina Pavic, Daniel Kwaku Abankwa
Formato: Artigo
Idioma:Inglês
Publicado: Elsevier 2024-12-01
Series:STAR Protocols
Assuntos:
Cell Biology
Cell-based Assays
Molecular Biology
Acceso en liña:http://www.sciencedirect.com/science/article/pii/S2666166724005136
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