Kinetic mechanism of Na+-coupled aspartate transport catalyzed by GltTk

Trinco et al. measure aspartate uptake rates in proteoliposomes containing purified prokaryotic Na+-coupled aspartate transporter GltTk. To overcome limitation of protein orientation, they use synthetic nanobody that blocks transporters from outside and reveal mechanistic features of Na+-aspartate s...

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Autors principals:	Gianluca Trinco, Valentina Arkhipova, Alisa A. Garaeva, Cedric A. J. Hutter, Markus A. Seeger, Albert Guskov, Dirk J. Slotboom
Format:	Artigo
Idioma:	Inglês
Publicat:	Nature Portfolio 2021-06-01
Col·lecció:	Communications Biology
Accés en línia:	https://doi.org/10.1038/s42003-021-02267-y
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