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An improved method for large-scale preparation of negatively and positively supercoiled plasmid DNA

A rigorous understanding of the biological function of superhelical tension in cellular DNA requires the development of new tools and model systems for study. To this end, an ethidium bromide–free method has been developed to prepare large quantities of either negatively or positively super-coiled p...

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Autors principals: Marita C. Barth, Debra A. Dederich, Peter C. Dedon
Format: Artigo
Idioma:Inglês
Publicat: Taylor & Francis Group 2009-07-01
Col·lecció:BioTechniques
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Accés en línia:https://www.future-science.com/doi/10.2144/000113172
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