Separation of plasmid DNA isoforms using centrifugal ultrafiltration
Centrifugal ultrafiltration is a well-established method for concentrating and purifying DNA. Here, we describe the use of centrifugal ultrafiltration for the separation of plasmid DNA isoforms based on differences in elongational flexibility of the supercoiled, open-circular, and linear plasmids. T...
Guardado en:
| Autores principales: | , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Taylor & Francis Group
2012-07-01
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| Colección: | BioTechniques |
| Materias: | |
| Acceso en línea: | https://www.future-science.com/doi/10.2144/0000113890 |
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