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Protocol to measure endogenous GlcNAc-1-phosphotransferase activity in SK-MEL-30 cells

Summary: GlcNAc-1-phosphotransferase (GNPT) is the key enzyme for tagging lysosomal hydrolases with the mannose 6-phosphate moiety for delivery to the lysosome. Here, we present the assay for measuring endogenous GNPT activity in SK-MEL-30 cells. We provide details for preparing the [3H]UDP-GlcNAc d...

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Principais autores: Balraj Doray, Danielle Henn, Xi Yang, Ming Li, Patricia Dickson
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2025-09-01
Serier:STAR Protocols
Fag:
Cell Biology
Molecular Biology
Protein Biochemistry
Online adgang:http://www.sciencedirect.com/science/article/pii/S2666166725003417
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