Protocol to measure endogenous GlcNAc-1-phosphotransferase activity in SK-MEL-30 cells
Summary: GlcNAc-1-phosphotransferase (GNPT) is the key enzyme for tagging lysosomal hydrolases with the mannose 6-phosphate moiety for delivery to the lysosome. Here, we present the assay for measuring endogenous GNPT activity in SK-MEL-30 cells. We provide details for preparing the [3H]UDP-GlcNAc d...
保存先:
| 主要な著者: | , , , , |
|---|---|
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Elsevier
2025-09-01
|
| シリーズ: | STAR Protocols |
| 主題: | |
| オンライン･アクセス: | http://www.sciencedirect.com/science/article/pii/S2666166725003417 |
| タグ: |
タグなし, このレコードへの初めてのタグを付けませんか!
|