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Targeted gene silencing in human embryonic stem cells using cell-penetrating peptide PepFect 14

Abstract Background Human embryonic stem (hES) cells serve as an invaluable tool for research and future medicine, but their transfection often leads to unwanted side effects as the method itself may induce differentiation. On the other hand, RNA interference (RNAi)-based targeted gene silencing is...

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Principais autores: Egle-Helene Ervin, Martin Pook, Indrek Teino, Valmar Kasuk, Annika Trei, Margus Pooga, Toivo Maimets
Format: Artigo
Sprog:Inglês
Udgivet: BMC 2019-01-01
Serier:Stem Cell Research & Therapy
Fag:
Human embryonic stem cells
siRNA transfection
Cell-penetrating peptide
PepFect 14
OCT4
B2M
Online adgang:http://link.springer.com/article/10.1186/s13287-019-1144-x
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