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Combined nanopore adaptive sequencing and enzyme-based host depletion efficiently enriched microbial sequences and identified missing respiratory pathogens

Abstract Background Enzyme-based host depletion significantly improves the sensitivity of clinical metagenomics. Recent studies found that real-time adaptive sequencing of DNA molecules was achieved using a nanopore sequencing machine, which enabled effective enrichment of microbial sequences. Howev...

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Principais autores: Mingyu Gan, Bingbing Wu, Gangfeng Yan, Gang Li, Li Sun, Guoping Lu, Wenhao Zhou
Format: Artigo
Sprog:Inglês
Udgivet: BMC 2021-10-01
Serier:BMC Genomics
Fag:
Metagenomics
Nanopore adaptive sequencing
Host depletion
Microbe enrichment
Online adgang:https://doi.org/10.1186/s12864-021-08023-0
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