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Single-molecule tracking of the transcription cycle by sub-second RNA detection

Transcription is an inherently stochastic, noisy, and multi-step process, in which fluctuations at every step can cause variations in RNA synthesis, and affect physiology and differentiation decisions in otherwise identical cells. However, it has been an experimental challenge to directly link the s...

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Principais autores: Zhengjian Zhang, Andrey Revyakin, Jonathan B Grimm, Luke D Lavis, Robert Tjian
Format: Artigo
Sprog:Inglês
Udgivet: eLife Sciences Publications Ltd 2014-01-01
Serier:eLife
Fag:
single-molecule
real-time
transcription
fluorescence
in situ hybridization
unstructured nucleic acid
Online adgang:https://elifesciences.org/articles/01775
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