A split protease-E. coli ClpXP system quantifies protein–protein interactions in Escherichia coli cells

Wang et al. developed a fluorescence-assisted single-cell methodology (split protease-E. coli ClpXP (SPEC)) to characterise protein-protein interactions for both eukaryotic and prokaryotic species in E. coli cells. Their method can quantify these interactions with high sensitivity, easy manipulation...

Ausführliche Beschreibung

Bibliografische Detailangaben

Hauptverfasser:	Shengchen Wang, Faying Zhang, Meng Mei, Ting Wang, Yueli Yun, Shihui Yang, Guimin Zhang, Li Yi
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Nature Portfolio 2021-07-01
Schriftenreihe:	Communications Biology
Online-Zugang:	https://doi.org/10.1038/s42003-021-02374-w
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