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Structure-guided protein engineering increases enzymatic activities of the SGNH family esterases

Abstract Background Esterases and lipases hydrolyze short-chain esters and long-chain triglycerides, respectively, and therefore play essential roles in the synthesis and decomposition of ester bonds in the pharmaceutical and food industries. Many SGNH family esterases share high similarity in seque...

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Main Authors: Zhengyang Li, Long Li, Yingyi Huo, Zijun Chen, Yu Zhao, Jing Huang, Shuling Jian, Zhen Rong, Di Wu, Jianhua Gan, Xiaojian Hu, Jixi Li, Xue-Wei Xu
Format: Artigo
Language:Inglês
Published: BMC 2020-06-01
Series:Biotechnology for Biofuels
Subjects:
Esterase
SGNH superfamily
Swapped structure
Alkaline adaptability
Online Access:http://link.springer.com/article/10.1186/s13068-020-01742-8
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