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Primer Design for PCR and Sequencing in High-Throughput Analysis of SNPs

To achieve high-throughput analysis of allele frequencies in human SNPs, we have developed automated methods for designing PCR and DNA sequencing primers. We found we could run the PCR assays at quite stringent, uniform conditions. The design process used freely available databases, including dbSNP,...

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Bibliografiske detaljer
Principais autores: Ellen F. Vieux, Pui-Yan Kwok, Raymond D. Miller
Format: Artigo
Sprog:Inglês
Udgivet: Taylor & Francis Group 2002-06-01
Serier:BioTechniques
Online adgang:https://www.future-science.com/doi/10.2144/jun0202
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