A Python script to design primers for overlap extension PCR to ligate two DNA fragments
Ligating two or more DNA fragments is a regular operation for the subcloning and the engineering of vectors. The overlap extension PCR serves as a straightforward method to solve this issue. However, it takes a relatively long time to design the appropriate overlapping primers and the primers for th...
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| Principais autores: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
PeerJ Inc.
2022-10-01
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| coleção: | PeerJ |
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| Acesso em linha: | https://peerj.com/articles/14283.pdf |
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