A Simplified Method for CRISPR-Cas9 Engineering of Bacillus subtilis
ABSTRACT The clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 system from Streptococcus pyogenes has been widely deployed as a tool for bacterial strain construction. Conventional CRISPR-Cas9 editing strategies require design and molecular cloning of an appropriate guide RNA (g...
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| Hauptverfasser: | , , |
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| Format: | Artigo |
| Sprache: | Inglês |
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American Society for Microbiology
2021-10-01
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| Schriftenreihe: | Microbiology Spectrum |
| Schlagworte: | |
| Online-Zugang: | https://journals.asm.org/doi/10.1128/Spectrum.00754-21 |
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