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Barriers to simultaneous multilocus integration in Bacillus subtilis tumble down: development of a straightforward screening method for the colorimetric detection of one-step multiple gene insertion using the CRISPR-Cas9 system

Abstract Background Despite recent advances in genetic engineering tools for effectively regulating and manipulating genes, efficient simultaneous multigene insertion methods have not been established in Bacillus subtilis. To date, multilocus integration systems in B. subtilis, which is one of the m...

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Principais autores: Jordi Ferrando, Oriana Filluelo, Daniel R. Zeigler, Pere Picart
Format: Artigo
Sprog:Inglês
Udgivet: BMC 2023-01-01
Serier:Microbial Cell Factories
Fag:
B. subtilis
CRISPR-Cas9
Colorimetric screening
Genome engineering
Multigene insertion
Online adgang:https://doi.org/10.1186/s12934-023-02032-2
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