Design and Construction of Portable CRISPR-Cpf1-Mediated Genome Editing in Bacillus subtilis 168 Oriented Toward Multiple Utilities

Bacillus subtilis is an important Gram-positive bacterium for industrial biotechnology, which has been widely used to produce diverse high-value added chemicals and industrially and pharmaceutically relevant proteins. Robust and versatile toolkits for genome editing in B. subtilis are highly demandi...

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Hlavní autoři:	Wenliang Hao, Feiya Suo, Qiao Lin, Qiaoqing Chen, Li Zhou, Zhongmei Liu, Wenjing Cui, Zhemin Zhou
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Frontiers Media S.A. 2020-09-01
Edice:	Frontiers in Bioengineering and Biotechnology
Témata:	CRISPR-Cpf1 Bacillus subtilis multiplex genome editing large fragment deletion gene insertion chassis microorganisms
On-line přístup:	https://www.frontiersin.org/article/10.3389/fbioe.2020.524676/full
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