Engineered CRISPR-Cas13a system with enhanced target RNA cleavage activity and reduced collateral activity for therapeutic applications

The CRISPR-Cas13 system exhibits potent RNA cleavage activity and has been widely utilized for RNA-targeting applications. However, its collateral cleavage of bystander RNAs limits in vivo therapeutic applications. In this study, we generated a series of LwaCas13a mutants through structure-based des...

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Hlavní autoři:	Wenxia Zhang, Haili Wang, Dongzi Liu, Xi Mao, Yi Zhang, Yimu Yang, Zehui Liu, Tong Pan, Yingle Liu, Qi Zhang
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Elsevier 2026-03-01
Edice:	Molecular Therapy: Nucleic Acids
Témata:	MT: Oligonucleotides: Therapies and Applications CRISPR-Cas13a crRNA cleavage activity collateral activity antiviral application
On-line přístup:	http://www.sciencedirect.com/science/article/pii/S2162253125003658
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