Mutant T4 DNA polymerase for easy cloning and mutagenesis.
The advent of high-fidelity DNA polymerases that can be used to linearize and amplify whole plasmids by PCR opened the door to greatly simplified cloning and mutagenesis protocols. Commercially available kits work well, but often have been optimized using undisclosed or proprietory components. Here...
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| Autores principales: | , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Public Library of Science (PLoS)
2019-01-01
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| Colección: | PLoS ONE |
| Acceso en línea: | https://doi.org/10.1371/journal.pone.0211065 |
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