A comparison of three DNA extractive procedures with Leptospira for polymerase chain reaction analysis
Three DNA extraction methods were evaluated in this study: proteinase K followed by phenol-chloroform; a plant proteinase (E6870) followed by phenol-chloroform; and boiling of leptospires in 0.1 mM Tris, pH 7.0 for 10 min at 100°C, with no phenol treatment. Every strain treated with proteinase K or...
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| Format: | Artigo |
| Sprache: | Inglês |
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Fundação Oswaldo Cruz (FIOCRUZ)
2000-01-01
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| Schriftenreihe: | Memorias do Instituto Oswaldo Cruz |
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| Online-Zugang: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000300008 |
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