A comparison of three DNA extractive procedures with Leptospira for polymerase chain reaction analysis

Three DNA extraction methods were evaluated in this study: proteinase K followed by phenol-chloroform; a plant proteinase (E6870) followed by phenol-chloroform; and boiling of leptospires in 0.1 mM Tris, pH 7.0 for 10 min at 100°C, with no phenol treatment. Every strain treated with proteinase K or...

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Detalhes bibliográficos

Principais autores:	Veloso IF, Lopes MTP, Salas CE, Moreira EC
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Fundação Oswaldo Cruz (FIOCRUZ) 2000-01-01
Colecção:	Memorias do Instituto Oswaldo Cruz
Assuntos:	polymerase chain reaction leptospira DNA extraction
Acesso em linha:	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000300008
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