Amplification-free detection of plant pathogens by improved CRISPR-Cas12a systems: a case study on phytoplasma

CRISPR-based disease detection has the potential to profoundly change how pathogens are detected in plant materials. However, there has been a lack of research directed into improving explicitly the CRISPR components that define these detection assays. To fill this technology gap, we have designed a...

ver descrição completa

Detalhes bibliográficos

Principais autores:	Joseph R. Lagner, Eric A. Newberry, Yazmín Rivera, Liyang Zhang, Christopher A. Vakulskas, Yiping Qi
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Frontiers Media S.A. 2025-03-01
coleção:	Frontiers in Plant Science
Assuntos:	CRISPR-Cas12a pathogen detection phytoplasma fluorescence based detection lateral flow assay (LFA) LbCas12a-ultra
Acesso em linha:	https://www.frontiersin.org/articles/10.3389/fpls.2025.1544513/full
Tags:	Adicionar Tag Sem tags, seja o primeiro a adicionar uma tag!