Amplification-free detection of plant pathogens by improved CRISPR-Cas12a systems: a case study on phytoplasma

CRISPR-based disease detection has the potential to profoundly change how pathogens are detected in plant materials. However, there has been a lack of research directed into improving explicitly the CRISPR components that define these detection assays. To fill this technology gap, we have designed a...

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Autores principales:	Joseph R. Lagner, Eric A. Newberry, Yazmín Rivera, Liyang Zhang, Christopher A. Vakulskas, Yiping Qi
Formato:	Artigo
Lenguaje:	Inglês
Publicado:	Frontiers Media S.A. 2025-03-01
Colección:	Frontiers in Plant Science
Materias:	CRISPR-Cas12a pathogen detection phytoplasma fluorescence based detection lateral flow assay (LFA) LbCas12a-ultra
Acceso en línea:	https://www.frontiersin.org/articles/10.3389/fpls.2025.1544513/full
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