New PAM Improves the Single-Base Specificity of crRNA-Guided LbCas12a Nuclease

The RNA-guided Cas12a nuclease forms a complex with a CRISPR RNA (crRNA) to cleave the double-stranded DNA target. Among others, Cas12a protein from <i>Lachnospiraceae bacterium</i> (LbCas12a) is widely used for biomedical research. For target recognition, LbCas12a requires a specific nucleotide seq...

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Bibliografiske detaljer

Principais autores:	Mariia A. Misiurina, Angelina V. Chirinskaite, Aleksandra S. Fotina, Andrey A. Zelinsky, Julia V. Sopova, Elena I. Leonova
Format:	Artigo
Sprog:	Inglês
Udgivet:	MDPI AG 2022-11-01
Serier:	Life
Fag:	LbCas12a protospacer adjacent motif collateral activity genotyping
Online adgang:	https://www.mdpi.com/2075-1729/12/11/1927
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