New PAM Improves the Single-Base Specificity of crRNA-Guided LbCas12a Nuclease
The RNA-guided Cas12a nuclease forms a complex with a CRISPR RNA (crRNA) to cleave the double-stranded DNA target. Among others, Cas12a protein from <i>Lachnospiraceae bacterium</i> (LbCas12a) is widely used for biomedical research. For target recognition, LbCas12a requires a specific nucleotide seq...
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| Autors principals: | , , , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
MDPI AG
2022-11-01
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| Col·lecció: | Life |
| Matèries: | |
| Accés en línia: | https://www.mdpi.com/2075-1729/12/11/1927 |
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